Effect of treatment with 5uM of the type I PRMT inhibitor MS023 dissolved in DMSO for 3 days and of a stably transduced PRMT1 specific dox-inducible knockdown over 4 days in ccRCC cell line models RCC243 and 786-0

To investigate the effect of pharmacological inhibition of the type I PRMTs in ccRCC cell lines. We treated one patient derived cell line (RCC243) and one commercially available line (786-0) in duplicate with 5uM MS023 for 3 days vs. 0.1% DMSO alone. We then performed gene expression profiling analysis using data obtained from RNA-seq. Each cell line was treated as a biological replicate. Similarly, to investigate the genetic knockdown of PRMT1 using the same cell lines, stably transduced dox-inducible PRMT1 targeting shRNA's. Cell lines were treated with or with out 1ug/mL dox for 4 days in triplicate. Gene expression profiling analysis was then performed using data obtained from RNA-seq. Overall design: Comparative gene expression profiling analysis of RNA-seq data for RCC243 and 786-0 treated vs untreated with 5uM MS023 for 3 days - Each cell line treated in duplicate and data pooled for analysis. Comparative gene expression profiling analysis of RNA-seq for RCC243 and 786-0 stably transduced with dox-inducible PRMT1 targeting shRNAs - Each cell line was dox induced (1ug/mL) in triplicate and comparison was made to non-induced controls. Cell line data pooled for analysis.