Transcription profiling by array of individual inner cells during mouse blastocyst development

Mammalian embryonic development begins with the specification and segregation of the two extra-embryonic lineages, trophectoderm and primitive endoderm, from the pluripotent embryonic lineage, the epiblast. To establish a map of epiblast (EPI) versus primitive endoderm (PrE) lineage segregation which occurs within the inner cell mass (ICM), we comprehensively characterised the gene expression profiles of individual inner cells during blastocyst development. Lineage represents the two embryonic cell lineages: the epiblast (EPI), and the primitive endoderm (PE), which are segregated within the inner cell mass (ICM) during blastocyst development.哺乳动物胚胎发育起始于两类胚外谱系——滋养外胚层（trophectoderm）与原始内胚层（primitive endoderm）——从作为多能胚胎谱系的上胚层（epiblast）的特化与分离过程。为构建囊胚内细胞团（ICM）中发生的上胚层（EPI）与原始内胚层（PrE）谱系分离图谱，我们对囊胚发育进程中单个内细胞的基因表达谱进行了全面表征。本研究所界定的谱系，特指囊胚发育过程中在内细胞团（ICM）内发生分离的两类胚胎细胞谱系：上胚层（EPI）与原始内胚层（PE）。