Genome-Wide Identification of Conditionally Essential Genes Supporting Streptococcus suis Growth in Serum and Cerebrospinal Fluid – Supplementary Dataset

This dataset contains supplementary data for the study “Genome-Wide Identification of Conditionally Essential Genes Supporting <em>Streptococcus suis</em> Growth in Serum and Cerebrospinal Fluid.” The research aimed to identify conditionally essential genes that facilitate <em>S. suis</em> survival in host environments. Using transposon sequencing (Tn-seq) combined with Oxford Nanopore sequencing, the study profiled mutant fitness in active porcine serum and cerebrospinal fluid derived from human choroid plexus organoids. The dataset includes Tn-seq output files, gene annotations, and raw and processed data supporting Figures 2 to 6 of the manuscript and Supplementary Tables S1 to S3. A detailed README file is included to describe the content and structure of each dataset component. The raw FAST5 and FASTQ sequencing files are available in the European Nucleotide Archive (ENA) under study accession number PRJEB89170. The Tn-Seq analysis pipeline developed for this study, Nanopore-TnSeq-Pipeline, is also available and can be accessed via a link provided in both the manuscript and this data repository.

本数据集为研究《全基因组鉴定支持猪链球菌（Streptococcus suis）在血清与脑脊液中生长的条件必需基因》提供补充数据。本研究旨在鉴定可帮助猪链球菌在宿主环境中存活的条件必需基因。本研究结合转座子测序（Tn-seq）与牛津纳米孔测序技术，对源自人类脉络丛类器官的活化猪血清及脑脊液中的突变体适应度进行了分析表征。本数据集包含转座子测序输出文件、基因注释文件，以及支撑论文手稿中图2至图6和补充表S1至S3的原始与处理后数据。数据集附带详细的README文件，用于说明各数据集组件的内容与结构。原始FAST5与FASTQ测序文件可在欧洲核苷酸档案库（ENA）中获取，研究登录号为PRJEB89170。本研究开发的转座子测序分析流程Nanopore-TnSeq-Pipeline同样可获取，可通过论文手稿与本数据仓库中提供的链接访问。