人类视网膜的单细胞分析确定了进化上保守的和物种特异性的发育控制机制--单细胞测序数据
收藏干细胞与再生医学数据中心2023-05-31 更新2024-03-06 收录
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资源简介：
获取胚胎发育期第12，14周的视网膜组织，将组织剪碎成小块，用木瓜蛋白酶和胶原酶IV以及DNase1将其消化成单细胞悬液，终止消化反应后进行流式分选，获取活性较好的视网膜单细胞，利用10x genomics对单细胞悬液进行扩增建库，获取单细胞文库，文库质检合格后送至测序公司使用Illumina平台测序，测序长度为双端150bp。
Retinal tissues were harvested from embryos at gestational weeks 12 and 14. The tissues were minced into small pieces, then digested into single-cell suspensions with papain, collagenase IV and DNase I. After terminating the enzymatic digestion, flow cytometric sorting was performed to obtain retinal single cells with favorable viability. Single-cell suspensions were amplified and used for library construction via the 10x Genomics platform to generate single-cell libraries. Following successful quality control (QC) of the libraries, they were sent to a sequencing company for sequencing on the Illumina platform, with a read length of 150 bp paired-end.
提供机构：
中国科学技术大学
创建时间：
2023-05-31
