Frequency of latently-infected cells and efficiency of reactivation.

1Data for IFNAR1-/- mice on either 129S2 or C57BL6/J backgrounds are shown grouped with the relevant wildtype mice for comparison. Data for IRF2-/- mice are shown grouped with IRF2+/+ littermates for comparison.
2Calculated by Poisson distribution from limiting dilution reactivation or viral genome PCR assays as described in Methods.
3Fold increase in reactivation is the ratio of reactivating cells of indicated mouse genotypes to reactivation observed in wildtype mice of the same genetic background infected with MHV68. Note that this ratio is not corrected for differences in viral genome frequency.
4P value is the value for statistical significance obtained comparing unmanipulated limiting dilution reactivation data sets (Figure 7) of ISREΔ to MHV68 in the same host genotype and at the same time post infection, as determined by Wilcoxon-matched pairs. N.S., not significant (p>0.05).
5Efficiency of reactivation is defined as the ratio of reactivating cell to latent cells. Note that this ratio is corrected for differences in viral genome frequency. An efficiency of one indicates that all latently-infected cells underwent reactivation. Efficiencies of greater than one indicate the maximal error inherent in detecting single viral genome positive cells as described in Methods.
6Where reactivation efficiency did not reach 63%, the frequency of cells reactivating was extrapolated from reactivation data sets assuming a sigmoidal dose-response curve with a Hill slope of 1.