A DNA methylation reader complex that enhances gene transcription

DNA methylation generally functions as a repressive transcriptional signal, but it is also? ?known to activate gene expression. In either case, the downstream factors remain? ?largely unknown. By using comparative interactomics, we isolated proteins in Arabidopsis? ?thaliana that associate with methylated DNA. Two SU(VAR)3-9 homologs, the transcriptional? ?antisilencing factor SUVH1, and SUVH3, were among the methyl reader candidates. SUVH1? ?and SUVH3 bound methylated DNA in vitro, were associated with euchromatic methylation in? ?vivo, and formed a complex with two DNAJ domain-containing homologs, DNAJ1 and DNAJ2.? ?Ectopic recruitment of DNAJ1 enhanced gene transcription in plants, yeast, and mammals.? ?Thus, the SUVH proteins bind to methylated DNA and recruit the DNAJ proteins to enhance? ?proximal gene expression, thereby counteracting the repressive effects of transposon? ?insertion near genes. Overall design: This dataset includes: 4 ATAC-seq, 26 ChIP-seq, 14 whole genome bisulfite seq (WGBS) and 45 RNAseq datasets.