Amplicon sequencing analysis of mcrA and pmoA of paddy soil in which rice inoculated with Azoarcus sp. KH32C was cultivated. rice paddy metagenome

The cultivation of rice inoculated with Azoarcus sp. KH32C mitigates methane emission from paddy field. To elucidate the soil microbial mechanisms of methane emission mitigation by KH32C inoculation, amplicon sequencing analyses of the methyl-coenzyme M reductase (mcrA) gene possessed by methanogens and particulate methane monooxygenase (pmoA) gene possessed by methanotrophs using complementary DNA were carried out.