Transcriptomic analysis of the abundance of snoRNAs and their host genes in 4 different cell lines, using low structure bias TGIRT-seq

Small nucleolar RNAs (snoRNAs) are highly abundant non-coding RNAs whose canonical function is to guide the 2'-O-ribose methylation or pseudouridylation of ribosomal RNAs. They are often encoded in longer genes referred to as their host genes, the expression of which is required for their own biogenesis. Due to their very stable structure, reverse transcription of snoRNAs using standard viral reverse transcriptases is very inefficient, and does not provide an adequate view of the abundance of these small RNAs. We thus employed high-throughput, low structure bias TGIRT-seq sequencing to quantified the abundance of the entire ribodepleted transcriptome, including snoRNAs and their host genes, in 4 different cell lines. Overall design: Ribodepleted and fragmented TGIRT-Seq librairies were generated from RNA samples originating from various cell lines with two biological replicates each.