Estrogen Signaling in Arcuate Kiss1 Neurons Suppresses a Sex-Dependent Circuit That Promotes Dense Strong Bones in Female Mice

Purpose: To characterize the estrogen receptor alpha (ESR1)-dependent transcriptome of neurons in the arcuate nucleus that regulate female bone density Method: Using RNA-Seq we compared gene expression profiles of microdissected arcuate nuclei from 3 control (Esr1fl/fl) and 3 conditional knockout (Esr1fl/fl;Nkx2-1-Cre) female mice.研究目的：解析调控雌性骨密度的弓状核神经元中，依赖雌激素受体α（estrogen receptor alpha, ESR1）的转录组特征。实验方法：本研究采用RNA-Seq技术，对比了3只对照（Esr1fl/fl）雌性小鼠与3只条件性敲除（Esr1fl/fl;Nkx2-1-Cre）雌性小鼠的显微切割弓状核的基因表达谱。