Gene expression after knockdown of transcription factors in human iPSC-derived cardiac myocytes

We report gene expression changes after knockdown of transcription factors in human iPSC-derived cardiac myocytes. In prior experiments we showed that transcription factors known to be important for cardiac development were frequently up-regulated (i.e., "responsive") after small molecule perturbations of cultured iPSC-derived cardiac myocytes. We used RNA sequencing to test whether siRNA-mediated knockdown of transcription factors with different perturbation-responsiveness and tissue-specificity profiles would lead to inappropriate up-regulation of non-myocyte gene sets in cardiac myocytes. Overall design: Paired-end RNA sequencing of 9 different targeting siRNA conditions, 1 scrambled siRNA control condition, and 1 non-transfected control condition. All conditions in triplicate. 33 total samples.