Transcriptome analysis of rhesus monkey germinal vesicle and failed-to-mature oocytes. Transcriptome analysis of rhesus monkey germinal vesicle and failed-to-mature oocytes

Oocyte maturation defects are one contributing factor in unexplained female infertility. Failure of some oocytes to undergo germinal vesicle breakdown or progress to second meiotic metaphase in response to an ovulatory stimulus can limit the number of high quality oocytes available for assisted reproduction. Understanding the molecular mechanisms that underlie oocyte maturation failure may lead to novel methods for increasing the number of high quality oocytes available for assisted reproduction, as well as providing new insight into the basic biology of oocyte maturation. Using RNA sequencing, we compared the transcriptome of rhesus monkey oocytes that failed to mature (FTM) in response to an ovulatory stimulus in vivo to normal germinal vesicle and metaphase II stage oocytes. Overall design: Transcriptomes of rhesus monkey oocytes that failed to mature (FTM) in response to an ovulatory stimulus in vivo, normal germinal vesicle, and metaphase II stage oocytes were compared using RNAseq.