Overview of the PPO family in dandelion.

aPredicted by the TargetP online tool [43]–[44]. C: predicted location is the Chloroplast; cTP: chloroplast transit peptide score, from 0 to 1 where 1 indicates the highest likelihood; RC: Reliability class, from 1 to 5, where 1 indicates the strongest prediction. Due to the twin-arginine motif (Figure 3) localization is predicted to be in the thylakoid lumen.bCalculated average molecular weight (MW) in kDa and isoelectric point (pI) of the mature protein (without the predicted transit peptide), computed by the Compute pI/Mw tool [45]–[46].cValues given in % sequence identity of full length protein sequences relative to PPO-1 (sequence identity matrix created by ClustalW2.1).dExpression was repeatedly tested by semi-quantitative RT-PCR; PPOs which were expressed in a given tissue in at least one experiment were assigned as “occasionally” expressed.