Dataset underlying the publication 'Optimization of Capture ELISAs for Chicken Cytokines Using Commercially Available Antibodies''

The research is about optimization and validation of capture ELISA for chicken IL-2, IL-6, IL-10, IL-12p40, and IFN-y using commercially available antibodies and recombinant cytokines. To optimize antibody concentrations, checkerboard titration was performed. Then, validation tests for capture ELISAs were performed to assess lower limit of detection (LLOD), matrix effect, parallelism, and inter-assay variation.

本研究以市售抗体与重组细胞因子为实验材料，针对鸡白细胞介素2（chicken IL-2）、鸡白细胞介素6（chicken IL-6）、鸡白细胞介素10（chicken IL-10）、鸡白细胞介素12p40（chicken IL-12p40）及鸡干扰素γ（IFN-γ）的捕获酶联免疫吸附试验（capture ELISA）的优化与验证工作展开研究。为优化抗体使用浓度，本研究采用棋盘格滴定法开展实验。随后针对该捕获酶联免疫吸附试验进行验证实验，以评估其检测下限（LLOD）、基质效应、平行性及批间变异。