Delivered complementation in planta (DCIP) enables measurement of peptide-mediated protein delivery efficiency in plants

Using DCIP, we demonstrate cell-penetrating peptide-mediated cytosolic delivery of peptides and recombinant proteins in Nicotiana benthamiana. We show that DCIP enables measurement of delivery efficiency and enables functional screening of cell-penetrating peptide efficacies for in-planta protein delivery. Finally, we demonstrate that DCIP detects cell-penetrating peptide-mediated delivery of recombinantly expressed proteins such as mCherry and Lifeact into intact leaves. We also demonstrate delivery of a recombinant plant transcription factor, WUSCHEL (AtWUS), into N. benthamiana. RT-qPCR analysis of AtWUS delivery in Arabidopsis seedlings also suggests delivered WUS can recapitulate transcriptional changes induced by overexpression of AtWUS. Taken together, our findings demonstrate that DCIP offers a new and powerful tool for interrogating cytosolic delivery of proteins in plants and highlights future avenues for engineering plant physiology.

Methods
The methods section of our manuscript describes how these data were collected and processed. They are organized in the figure structure of the manuscript. These zipped files contain:

Raw images (.czi) that were analyzed in our manuscript.
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The raw qPCR amplification data (.xlsx) used in our manuscript in the numerical source data folder to facilitate download. 
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