DNA methylation in rhabdomyosarcoma PDX and PDX-derived primary cells

"Conditions to maintain primary tumor cells in culture are largely unknown for most tumor entities including rhabdomyosarcoma. In this collaborative study with Dr. Beat Schafer's laboratory, we performed DNA methylation profiles of rhabdomyosarcoma patient-derived xenograft (PDX) and their matching PDX-derived primary cells (PPC), where the PPCs were developed and maintained under optimized culture conditions. We further compared DNA methylation profiles between these rhabdomyosarcoma PDXs and PPCs. This comparison may help us to evaluate a feasibility of in vitro profiling of primary rhabdomyosarcoma cells from a perspective of DNA methylation."