Expression data for mouse embryogenesis from oocyte to newborn

Studies in mouse have led to enormous progress in our understanding of early human development. The identification of genes and the signaling pathways involved in mouse embryogenesis have helped us to better understand fertilization, morulation, gastrulation, organogenesis and embryonic development in mammals. We report a detailed analysis of the global gene expression profiles from oocyte to the end of organogenesis in mouse. Our studies revealed distinct temporal regulation patterns for genes belonging to different functional categories, supporting their roles during organogenesis. Mouse embryos were selected at successive stage for for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain homogeneous populations of embryos at each developmental stage in order to increase the temporal resolution of expression profiles. To that end, we hand-selected embryos according to morphological criteria at 12 time-points from embryos to newborn以小鼠为模型的相关研究极大推动了人类对早期发育的认知。对小鼠胚胎发生过程中涉及的基因与信号通路的鉴定工作，助力我们更深入地理解哺乳动物的受精、桑椹胚形成、原肠胚形成、器官发生及胚胎发育进程。本研究针对小鼠从卵母细胞阶段至器官发生结束阶段的全基因组基因表达谱展开了详细分析。研究结果显示，不同功能类别的基因呈现出独特的时序调控模式，这为其在器官发生过程中的功能角色提供了佐证。我们按连续发育阶段选取小鼠胚胎，用于RNA提取及Affymetrix微阵列杂交实验。为提升基因表达谱的时间分辨率，我们力求在每个发育阶段获取均一化的胚胎群体。为此，我们依据形态学标准，在从胚胎发育至新生小鼠的12个时间节点中手工挑选胚胎。