High salinity-induced changes in metabolite levels.

The metabolite contents of rosette leaves of 4 week old, soil-grown Arabidopsis thaliana were determined upon watering with 150 mM NaCl. Pools of ten individual leaf rosettes were analysed four times using GC-MS. Compounds were identified by their retention index (TAG RI), quantitative mass tag (TAG MASS) and reverse match value (REV MATCH) of the mass spectra comparison. Ascorbic acid and dehydroascorbic acid levels were determined by HPLC and starch levels were quantified spectrophotometrically. Values are x-fold changes compared to the corresponding unstressed controls. Bold letters indicate significant changes of metabolites (t-test; P-value <0.05).