Integrated omics analysis on the bacterial community in the anaerobic digestion of chicken manure materials in biogas production farm

The overall effectiveness of anaerobic digestion is affected by the initial three phases as a result of substrates created for the methanogens to take in. In this study, the bacterial composition of the various chambers in the anaerobic digestion of chicken manure was investigated. Diversity of the microbial community for chicken manure was relatively simpler, consisting mainly of Lactobacillus, compared to the main digestion chambers with a more complex community. Chicken manure only has hydrolysis phase occurring, while the main digesters have the first three phases of anaerobic digestion concurrently occurring. A comparison of the metagenomics versus metaproteomics analysis revealed that the main difference was in the percentage of unknown microorganisms. The quantity of volatile fatty acids detected in each respective chamber suggested the effectiveness of the microorganisms in assimilating the metabolites. Successful integration of these study modes would present a better understanding on anaerobic digestion of complex systems.