miR-K5 expression and inhibition. Homo sapiens

Transcriptional profiling of BJAB cells expressing miR-K5 and BCBL cells treated with miR-K5 inhibitor. To identify host RNA targets of KSHV miRNAs, we took advantage of the observation that RNAs targeted by miRNAs often display small reductions in their steady-state levels, perhaps as a result of their impaired translation. Accordingly, we examined cellular transcript accumulation by array-based expression profiling under four sets of conditions in which KSHV miRNAs were expressed or inhibited. Keywords: Experimental treatment versus control treatment Overall design: Cells transfected with negative control miRNA compared to miR-K5 or negative miRNA inhibitor compared to miR-K5 inhibitor or stable cells transduced with empty vector compared to stable cells transduced to express miR cluster K1,2,3,4,5.