Raw data of flow cytometric analyses from Himadri B. Thapa et al., Enrichment of human IgA-coated bacterial vesicles in ulcerative colitis as a driver of inflammation, Nat Commun. 2025. Fig. 3A+B, Sup. Fig. 4A+6A.

Figure 3A: Flow cytometric analysis of human intestinal organoids testing for CD89 expression. (Antibody conjugates: CD89-PE) Colonic organoids were developed from organoid cultures derived from human biopsies. Human monocytes of 3 individual donors were used as positive control for CD89 expression in comparison. (Antibody conjugates: CD14-AF700, CD11b-PE-Cy7 and CD89-PE) Analysis was performed using a Beckman CytoFLEX Flow Cytometer. Figure 3B: Human monocytes isolated from peripheral blood via magnetic sorting using the Miltenyi CD14 MicroBeads (#130-050-201) are positive for CD14, CD11b and CD89 expression, n=4. (Antibody conjugates: CD14-AF700, CD11b-PE-Cy7 and CD89-PE) Data was recorded with a BD Bioscience LRS Fortessa. Sup.Fig. 4A: Flow cytometric analysis of U937 cells treated with EtOH solvent control, vitamin D3 (VD3) alone, TGF-β1 (TGF-β1) alone or with VD3 + TGF-β1 in combination for 48h for CD14, CD11b and CD89 expression. Only U937 cells treated with VD3 + TGF-β1 are CD14+CD11b+CD89+, n=3. (Antibody conjugates: CD14-AF700, CD11b-PE-Cy7 and CD89-PE) Data was recorded with a BD Bioscience LRS Fortessa. Sup.Fig. 6A: Flow cytometric analysis of Bone Marrow-Derived Dendritic Cells (BMDCs) prepared from CD89wt/wt (Littermate) and CD89tg/wt (CD89-transgenic) mice for CD89 expression. Viability was determined using a 7-AAD dye (negative cells are considered alive), n=15. (Antibody conjugate: CD89-PE) Data was recorded with a BD Bioscience LRS Fortessa. Gating strategies for all the data can be found in the original publication. Data analysis was performed with FlowJo 10. The publication related to this data can be found here: Thapa, H.B., Passegger, C.A., Fleischhacker, D. et al. Enrichment of human IgA-coated bacterial vesicles in ulcerative colitis as a driver of inflammation. Nat Commun 16, 3995 (2025). https://doi.org/10.1038/s41467-025-59354-5