List of strains, plasmids, cosmid and BACs used in this work.

abla, ampicillin resistance gene; neo, kanamycin resistance gene; aac(3)IV, apramycin(apra) resistance gene; oriT, origin of transfer; aadA, spectinomycin/streptomycin (spec) resistance gene; gam, inhibitor of the host exonuclease V; bet, single-stranded DNA binding protein; exo, exonuclease promoting recombination along with bet; cat, chloramphenicol resistance gene; attPφC31, φC31 attachment site from the φC31 phage; intφC31, integrase gene of φC31; flp, FLP recombinase gene; repA101, thermosensitive replication origin; xis and int: excisionase and integrase of pSAM2, respectively.