Porcine reproductive and respiratory syndrome virus type 2 hijacks the host cap-dependent translation system to prioritize viral replication

We identified host proteins interacting with PRRSV-2 N protein through co-immunoprecipitation (Co-IP) coupled with LC-MS/MS analysis. HA-tagged N protein was expressed in HEK293T cells, lysed with NP-40 buffer, and immunoprecipitated using anti-HA magnetic beads (empty vector controls were included). The purified complexes were co-incubated with porcine alveolar macrophages (PAMs) lysates (Fig. 1A). Following this, LC-MS/MS analysis was performed on complexes pulled down by anti-HA magnetic beads binding N protein from lysates of PAM cells, alongside control pull-downs (Fig. 1A).