Analysis of gene expression in OVA-specific tolerant T cell and naive T cell after stimulation with OVA.

In this study, in order to analyze molecular mechanism for serial anergy transfer, we compared specific gene expressed on the anergy T cells compared with naïve T cells after both are co-culture with OVA. Microarray analysis showed extremely higher expression of TIGIT in the anergy T cells than naïve T cells in the presence of OVA. Moreover, when naïve T cells and anergy T cells are co-culture with OVA, the naive T cells showed the increase of TIGIT+ T cells in comparison to activated naïve T cells. In conclusion, TIGIT+ anergy T cells induce a suppressor function to naïve T cells in a cell to cell contanct-dependent manner. Gene expression in naïve T cell cocultured with anergic T cell and naïve CD4 T celll was measured at 24 hours after stimulation of OVA