Effects of Nogo-A deletion on the trigeminal ganglion

Nogo-A is a major regulator of neural development and regeneration in the central nervous system, but its role in tooth innervation remains largely unknown. Neurons of the trigeminal ganglion innervate the teeth. We showed that Nogo-A is expressed in the trigeminal ganglion and tooth-related nerve fibres. Nogo-A deletion in mice leads to a less complex neuronal network when compared to wild-type animals. Bulk RNA sequencing on the trigeminal ganglia of Nogo-A KO and wild-type mice revealed gene expression changes associated with alterations in neurotrophin signalling and neuronal synaptic formation during the development and maturation of the trigeminal neurons. Overall design: The trigeminal ganglia were isolated from wild-type and Nogo-A KO mice at embryonic day 14 (E14) and postnatal day 25 (P25). Total RNA was extracted and sequenced. n = 4 wild-type and n = 4 Nogo-A KO animals were analysed for each stage; each sample contains one trigeminal ganglion.