RNA-seq on periodontal tisuues of susceptible and resistant collaborative cross mice after periodontal infection

We assessed alveolar bone changes of Collaborative cross (CC) lines in response to a mixed oral infection with a well-known periodontal pathogen. The CC lines showed significant variation in their response to the mixed infection. We then carried out RNA-seq analysis on periodontal tisuues of resistant CC lines, susceptible CC lines and two CC lines that showed bone formation after oral bacterial infection. Overall design: We assessed alveolar bone changes of Collaborative cross lines after periodontal infection with P.g. (strain 381) and F.n. (strain PK1594) using micro–computed tomography (µCT) (control groups were treated with PBS and 2% Carboxymethycellulose). The CC lines showed significant variation in their response to the mixed infection. We then carried out RNA-seq analysis on periodontal tisuues of two resistant CC lines (TAU-IL188, TAU-IL111), two susceptible CC lines (TAU-IL785, TAU-IL551) and two CC lines that showed bone formation after oral bacterial infection (TAU-IL2124, TAU-IL2126).