Cross-platform Clinical Proteomics using the Charité Open Standard for Plasma Proteomics (OSPP)

we present the Charité Open Peptide Standard for plasma proteomics (OSPP), an open resource composed of 211 isotope-labeled peptides, intended to be used as an internal standard for plasma and serum proteomic projects. The OSPP was designed based on peptides consistently quantified across a panel of diverse human studies, is made of peptides that can easily be synthesized, that distribute equally over chromatographic gradients, and that show consistent identification performance across diverse LC-MS platforms, acquisition methods, and matrices.