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Expression matrices of circRNAs and coding genes from siFOXA1 and control group in the HepG2 cells

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE161174
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NOT PROVIDED; REQESTED The Agilent Human circRNA Microrray V6 (4*180K,Design ID:084410) was used in this experiment and data analysis of the 2 samples have been completed. Total RNA was quantified by the NanoDrop ND-2000 (Thermo Scientific)and the RNA integrity was assessed using Agilent Bioanalyzer 2100 (Agilent Technologies).The sample labeling, microarray hybridization and washing were performed based on the manufacturer’s standard protocols. Briefly, total RNA were transcribed to double strand cDNA, then synthesized into cRNA and labeled with Cyanine-3-CTP. The labeled cRNAs were hybridized onto the microarray. After washing, the arrays were scanned by the Agilent Scanner G2505C(Agilent Technologies).
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2021-02-16
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