scRNA-seq analysis of murine MBC subsets

Purpose: We wanted to explore further heterogeneity of MBC subsets that were previously defined by our lab. Methods: MBC subsets were sorted and stained with unique anti-mouse HTO antibodies (CITE-seq). Results: We found that heterogeneity among MBC subsets was most prominent among DP MBC subsets, which could be separated by extrafollicular or germinal center transcriptional signatures, whereas DN and SP MBCs mostly exhibited an extrafollicular signature. Overall design: MBCs were generated using our novel Balb/c transfer system. At 10 weeks, MBC subsets were sorted, assigned HTOs, and sequenced using Illumina NovaSeq 6000