Cottonseed Vicilin Proteins: Potential Allergen Risk and Source of Bioactive Peptides

Glandless cottonseed
(Glc) is an exciting new food source but contains
C72 and GC72A vicilin proteins similar to the Ara h 1 peanut allergen.
The objective here was to evaluate some of the immunologic, catabolic,
and bioactive peptide properties of the C72 and GC72A proteins. Immunoblot
assay indicated that C72 and GC72A were recognized by three anti-Ara
h 1 antibodies. Enzyme-linked immunosorbent assay (ELISA) with purified
native C72 and GC72A showed they were recognized by IgE from 13 of
25 (∼50%) peanut or tree-nut-allergic sera. A subset of these
sera also recognized recombinant C72 and GC72A. In vitro simulated digestion indicated that Glc proteins were readily digested.
In silico analysis revealed that C72, GC72A, and other cupin domains
containing Glc proteins are potential sources of bioactive peptides.
The results presented here indicate that the C72 and GC72A proteins
represent an allergen concern and potential source of bioactive peptides
in food applications.