Differential expressions of exosomal miRNA from HMEC-1 cells which treated with cyclic higher pressure

The project aims to investigate the effect of cyclic higher pressure treatment on the expression of microRNA in exosomes secreted by human microvascular endothelial cells (HMEC-1), so as to provide a molecular mechanism basis for elucidating relevant physiological and pathological processes. The experiment divides HMEC-1 cells into two groups: Group C (normal culture without cyclic higher pressure, serving as control) and Group CHP (treated with cyclic higher pressure). After the cells are cultured to a specific stage, the culture media of both groups are collected, and exosomes are extracted from the combined samples of 6 plates. The expression profiles of microRNA in them are detected by high-throughput sequencing to identify the differential expressions of exosomal miRNA between the two groups.