Whole esophagi of WT, Nrf2-/- and Keap1-/- mice on C57BL background at E11.5, E15.5, P0 and P7, and esophageal epithelium of adult (8 weeks old) mice

Transcriptional profiling of mouse esophageal development. Goal was to globally profile critical genes and signaling pathways during the development of mouse esophagus and determine how Nrf2/Keap1 pathway regulates the morphogenesis of the esophageal epithelium. Multiple-comparison. WT-E11.5 vs. WT-E15.5 vs. WT-P0 vs. WT-P7; WT-P7 vs. WT-adult; WT-adult vs. Nrf2-/--adult; WT-P7 vs. Nrf2-/--P7 vs. Keap1-/--P7 vs. Nrf2-/-Keap1-/--P7. Biological replicates: 3 replicates for each group.本数据集为小鼠食管发育的转录组表达谱分析（Transcriptional profiling）。研究目标为全局解析小鼠食管发育进程中的关键基因与信号通路，并明确核因子红细胞系2相关因子2/Kelch样ECH关联蛋白1（Nrf2/Keap1）通路对食管上皮形态发生的调控作用。本次分析采用多重比较实验设计，具体对比分组包括：野生型（Wild Type, WT）胚胎第11.5天（E11.5）、E15.5、出生后0天（P0）与出生后7天（P7）样本；WT-P7与WT成年小鼠样本；WT成年小鼠样本与Nrf2基因敲除成年小鼠（Nrf2-/-adult）样本；WT-P7、Nrf2基因敲除出生后7天小鼠（Nrf2-/-P7）、Keap1基因敲除出生后7天小鼠（Keap1-/-P7）以及Nrf2与Keap1双基因敲除出生后7天小鼠（Nrf2-/-Keap1-/-P7）样本。所有实验组均设置3次生物学重复。