Combined effects of thermal environment and gene regulation on metabolic enzyme activities of major metabolic tissues in a winter-breeding amphibian

DATA001_mass.csv: Snout-vent length, wet mass of body, kidney, and liver of 18 male adult <i>Leptobrachium liui</i>. BMB: Pre-acclimation body mass (g); BMA: Post-acclimation body mass (g); KWM: Kidney wet mass (g); LWM: Liver wet mas (g)s; SVL: Snout-vent length (mm). For detailed results of the descriptive statistics, please refer to Table S4.DATA002_all.csv: metabolic enzyme activities and related gene expression in different tissues of adult male <i>Leptobrachium liui</i>. enzyme_CCO: cytochrome c oxidase activity (U/g port); enzyme_LDH: lactate dehydrogenase activity (U/g port); enzyme_SDH: succinate dehydrogenase activity (U/g port); gene_cox1: cox1 relative expression; gene_cox2: cox2 relative expression; gene_cox3: cox3 relative expression; gene_ldha: ldha relative expression; gene_sdha: sdha relative expression. LG_ means data was transformed by log10.water_temperature_4.5.6.7.11.csv: The stream water temperatures of Zhejiang Jiulongshan Natioanl Nature Reserve in April, May, June, July, and November 2021. detail in Fig. S1.water_parameters.csv：Water quality parameters data from nest sites of <i>Leptobrachium liui</i> during the field breeding season and the thermal acclimation experiment.kidney_LGgene.csv: Data for one-way ANOVA to compare different treatments.liver_LGgene.csv: Data for one-way ANOVA to compare different treatments.Coding.R: R code used for data analysis in this study.<br>