Effect of rosiglitazone treatment on insulin sensitivity in adipose tissue of type 2 diabetic patients

The goal of this study was to analyse the effect of a 12 weeks treatment with rosiglitazone on insulin sensitivity in the adipose tissue of type 2 diabetic patients. Diabetic patients were submitted to a 3 hours hyperinsulinemic- euglycemic clamp. Adipose tissue biopsies were taken before and after the clamp, labelled A & B respectively. Then, the patients were treated with rosiglitazone, agonist of PPAR gamma, during 12 weeks. After the treatment, all the patients were submitted to a second 3 hours hyperinsulinemic-euglycemic clamp. Adipose tissue biopsies were taken before and after the clamp, labelled C & D respectively. Adipose tissues Biopsies from 7 diabetic patients were used in this study. The samples from the same patients (obtained before and after the hyperinsulinemic-euglycemic clamp) were compared on the same microarray. Biopsie taken before the clamp was considered as the control. A dye switch protocol was applied.