NAIL: an evolutionarily conserved lncRNA essential for coordinating p38 activation and NF?B mediated chromatin remodelling in inflammation_3

We report the differential gene expression analysis in MCF7-NAILWT and MCF7-NAIL?NF?B cells stimulated with TNF alpha for 0min, 45min and 90 min. Overall design: MCF7-NAILWT and MCF7-NAIL?NF?B cells (WT and NAIL?NF?B cells were generated by Crispr/Cas9 genome editing by targeting the 2 NF?B sites in the promoter of NAIL. (Loc105375914) gene. Cells were stimulated with TNFalpha (10ng/ml) for 0, 60 and 120 mins. Total RNAs were isolated using Trizol. RNA-Seq library was prepared by Illumina Truseq Total RNA sequencing kit according to manufacturer instructions. Paired-end sequencing was performed using Illumina Hiseq, 2x101bp. Raw reads were aligned to mouse genome mm10 by Tophat2 .