supplemental data AJP-renal.pdf

<b>S1. </b>V-ATPase E1 subunit (red, arrows) expression to localize ICs and Aqp2 (green) expression to localize collecting ducts. WT^kidney&gt;WT^recipientkidneys (<b>A</b>) appeared to have similar number of intercalated cells (red cells, arrows) compared to WT native kidneys (<b>B</b>). <i>Car2^-/-</i>^kidney&gt;WT^recipientkidneys (<b>C)</b>appeared to have similarly diminished numbers of ICs (red cells, arrows) compared to <i>Car2</i>^-/-native kidneys (<b>D</b>). On A, the cells were stained with the inverse secondary colored and were pseudocolored red or green for consistency with the other images. <b></b> <b><br></b><b>S2. </b>Transplant hydronephrosis in excluded mouse<b></b><b> </b><b><br></b><b>S3. </b>Murine<b></b>VCUG demonstrating no vesicoureteral reflux status post renal transplant <b><br></b><b>S4</b>. Complete results of RT²Profiler Bacterial Response PCR Arrays evaluating acetazolamide vs. no acetazolamide in the kidney at baseline. <b><br></b><b>S5</b>. Complete results of RT²Profiler Bacterial Response PCR Arrays evaluating acetazolamide vs. no acetazolamide in the kidney during UTI at 6-hrs post transurethral UPEC inoculation. <b><br></b><b>S6</b>. Primer sequences for RT-PCR.