Long-term multichannel recordings in Drosophila flies reveal altered predictive processing during sleep compared with wake

During sleep, behavioral responsiveness to external stimuli is decreased. This classical definition of sleep has been applied effectively across the animal kingdom to identify this common behavioral state in a growing list of creatures, from mammals to invertebrates. Yet it remains unclear whether decreased behavioral responsiveness during sleep is necessarily associated with decreased responsiveness in brain activity, especially in insects. Here, we perform long-term multichannel electrophysiology in tethered Drosophila melanogaster flies exposed continuously to repetitive visual stimuli. Flies were still able to sleep under these visual stimulation conditions, as determined by traditional immobility duration criteria for the field. Interestingly, we did not find any difference between responses to repetitive visual stimuli during sleep compared to wake when we recorded local field potentials (LFP) across a transect of the fly brain from optic lobes to the central brain. However, we di..., , # Long-term multichannel recordings in Drosophila flies reveal altered predictive processing during sleep compared with wake

Dataset DOI: [10.5061/dryad.7pvmcvf5h](10.5061/dryad.7pvmcvf5h)

## Description of the data and file structure

### Data processing and analysis scripts

* LFP analysis script can be found on the associated Github ([https://github.com/MNVDP/MultichannelLFPAnalysis/tree/main](https://github.com/MNVDP/MultichannelLFPAnalysis/tree/main)), under the \"LFP Processing\" subdirectory

### Data types

* LFP data is stored in datestamped (e.g., \"060721\") folders as .mat files, to be loaded by the LFP analysis script
* Processed behavioural data (for overnight experiments) is also stored as .mat files, again to be loaded by the LFP analysis script

### Folder structure

* \[root folder (e.g. \"\LFP\\\")]
* \Scripts\ 
* \[[LFP analysis script (e.g. \"OddballProcessing 14point25 releaseBranch\") from the Github]]
* \toolboxes\
* \[various functions supplied with th...,