Whole-Genome Sequencing and Annotation of Barrientosiimonas humi gen. nov., sp. nov. 39T, a novel rare actinobacteria from Barrientos Island, Antarctica. Barrientosiimonas humi gen. nov., sp. nov. 39T

A genomic analysis study presents the whole-genome sequencing and annotation of Barrientosiimonas humi gen. nov., sp. nov. 39T to unravel the genomics data of B. humi sequences and understand the related biological functions information expression. Whole genome of B. humi isolate was sequenced using PacBio Sequel and then polished by Illumina HiSeq. The data was further undergone bioinformatic analysis for genome annotation. The circular genome of B. humi was then visualized. Totally, 83,639 reads were predicted from its 3.6Mb genome size. It composed of 3,381 coding genes, with about 95% were functionally annotated. B. humi was evident sharing close sequence similarity with species Demetria terragena and family Dermacoccaceae. Gene Ontology (GO) indicated cell and cell part were highly represented among cellular component, catalytic activity and binding were most enriched process within molecular function, metabolic process and cellular process were the top in biological process. Clusters of Orthologous Group (COG) revealed metabolism related genes were highly enriched, mostly were mapped to amino acid transport metabolism, transcription, energy production and conversion. Kyoto Encyclopedia of Genes and Genomes (KEGG) reported that metabolism process was the most represented KEGG pathways, which biosynthesis of antibiotics and metabolic pathways associated genes were significantly functioning. There were 52 biosynthetic gene clusters involved in secondary metabolites biosynthesis. We found that B. humi was capable to produce antibacterial, antifungal, cytotoxic and inhibitor bioactive compounds. The natural producer of multiple ectoine biosynthesis genes were expressed in B. humi genome region, which elucidating its potential to adapt and function in extreme environments. B. humi genomic sequence positions of the predicted coding regions were illustrated. Our research is the first whole-genome sequencing and annotation study in B. humi, which serves as a significant approach that offers comprehensive genomic data to accelerate the future studies of novel secondary metabolites extraction for innovative natural antibiotics and cancer drugs.