Correlated responses to selection across diverse environments during experimental evolution of <em>Tetrahymena thermophila</em>

Correlated responses to selection have long been observed and studied; it remains unclear when they will arise, and in what direction. To contribute to a growing understanding of correlated responses to selection, we used the experimental evolution of the ciliate&nbsp;Tetrahymena thermophila to study direct and correlated responses in a variety of different environmental conditions. One experiment focused on adaptation to two different temperatures and the correlated responses across temperatures. Another experiment used inhibitory concentrations of a variety of compounds to test direct and correlated responses to selection. We found that all populations adapted to the environments in which they evolved. We also found many cases of correlated evolution across environments; few conditions resulted in tradeoffs, and many resulted in a positive correlated response. Surprisingly, in many instances, the correlated response was of a larger magnitude than the direct response. We find that ance..., , , # Data from: Correlated responses to selection across diverse environments during experimental evolution of *Tetrahymena thermophila* [https://doi.org/10.5061/dryad.vx0k6djzb](https://doi.org/10.5061/dryad.vx0k6djzb) ## Description of the data and file structure Growth curves were collected using 96-well plates and a microplate reader. * ms3_generations_3875-4125.xls contains the data from the 3 genotype 2 temperature experiment. The mean growth rate after 4000 generations is given for each genotype in each evolution and assay condition. Columns show the evolution temperature, the assay temperature (degree C), the population, the initial genotype, the 250-generation bin over which the mean growth rate is calculated, the number of growth rates measured from which the mean is calculated, the mean growth rate, the ancestral growth rate, the relative increase in growth rate, and the absolute change in growth rate. - growth_parameters_in_home_vs_away_environment_050824.xls contains the ...,