Endothelial TGFb signaling drives vascular inflammation and atherosclerosis [ChIP-Seq]

Endothelial TGFβ signaling is one of the primary drivers of atherosclerosis-associated vascular inflammation.  Inhibition of endothelial TGFβ signaling in hyperlipidemic mice reduces vessel wall inflammation and vascular permeability and leads to arrest of disease progression and regression of established lesions. We performed scRNAseq method to examine endothelial cell gene expression profile using Apoe and EC specific TGFbR1/2 KO in Apoe background mice. Examination of endothelial cell TGFb signaling downstream transcription factor Smad2/3 in the regulation of inflammatory gene promoters.