Microbiota profile in lung tissue of tuberculosis and non-tuberculous pulmonary diseases based on metagenomic next-generation sequencing and immune-related transcriptional profile revealed by transcriptome sequencing

Lung microbiota was analyzed based on metagenomic next-generation sequencing (mNGS) data in lung tissues from 110 patients with tuberculosis (TB) and 25 with non-tuberculous (NonTB) pulmonary diseases. Transcriptome sequencing was carried out in tuberculosis tissues (TB, n=3), adjacent tissues to tuberculosis (ParaTB, n=3), and non-tuberculosis tissues (NonTB, n=3).