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Figure 7. Effects of polymicrobial biofilm on the (A) exoprotein production in monomicrobial biofilm and polymicrobial biofilm with (B) protein abundance quantified by the area under the curve (AOC) analysis and the effects on gene expression of key virulence factors and two-component system of P. aeruginosa (C). The effects of cell-free conditioned media (CFCM) on S. aureus virulence gene expression after 24 hours of treatment (D). Statistical analysis was conducted using an ordinary one-way ANOVA with the Dunnett post hoc test with 95% (p < 0.05) of signif-icance. *: p < 0.05; **: p < 0.001; ns, not significant.