Single base substitution mutational signatures in pediatric acute myeloid leukemia based on whole genome sequencing

This dataset includes whole genome sequencing (WGS) data of 20 diagnostic, and 20 remission samples from 20 children/adolescents with acute myeloid leukemia (AML), treated at the Departments of Pediatrics at Lund and Linköping University Hospitals between 1994 and 2016. The median age of the patients was 8 years (range 0-17 years) and the female/male ratio was 1:1. DNA was extracted from diagnostic bone marrow (BM; n = 17)/peripheral blood (PB; n = 3) samples, remission BM (n = 15)/PB (n = 5), and from two BM relapses. Construction of libraries, using the TruSeq Nano DNA sample preparation kit (Illumina, San Diego, CA, USA) on 100 ng DNA, and massively parallel sequencing were performed by BGI Tech Solutions (Hong Kong). The WGS (Illumina HiSeqX) reached an average sequencing depth of 30-42x/sample (median 33x), with 2x 150 bp read length.本数据集包含1994年至2016年间，于隆德大学医院与林雪平大学医院儿科接受治疗的20例急性髓系白血病（AML）儿童/青少年患者的20份诊断样本与20份缓解期样本的全基因组测序（WGS）数据。患者中位年龄为8岁（年龄范围0~17岁），男女比例为1:1。DNA提取自诊断样本的骨髓（BM，n=17）/外周血（PB，n=3）、缓解期样本的BM（n=15）/PB（n=5），以及2份BM复发样本。文库构建采用TruSeq Nano DNA样本制备试剂盒（Illumina，美国加利福尼亚州圣地亚哥），以100 ng DNA为起始材料，高通量测序工作由BGI Tech Solutions（香港）完成。本数据集的WGS（Illumina HiSeqX）平均测序深度为30~42×/样本（中位深度33×），读长为2×150 bp。