Unr is a potential post-transcriptional master regulator of protein expression

Ribonucleoprotein immunoprecipitation followed by mass spectrometry and RNA-Seq was used to investigate the ‘Upstream of N-RAS’ (UNR) protein interactome across three cancer cell lines. A number of novel UNR-interacting proteins and transcripts were discovered, including the ubiquitin E3 ligase, HUWE1; the nucleolar protein, NARR; the multifunctional protein, SQSTM1; and the erythropoiesis-related protein, LDB1 . GO-term analysis of putative UNR interactors suggested that UNR interacts with both proteins and mRNAs encoding proteins that are involved in RNA binding Examination of UNR binding to RNA by RIP-RNA-Seq using three replicates of three cell types,