Regulation of ecfF in a B. japonicum ΔosrA background complemented with wild-type or mutant versions of osrA.

aCells were grown micro-oxically without stress or were exposed to 2 mM H2O2 for 10 min prior to harvest. Expression levels of ecfF in different backgrounds were determined by qRT-PCR and expressed as fold-change values ± standard errors relative to the expression detected in the pseudo wild-type strain 92-29 under non-stress conditions. Data are based on three technical replicates of a representative experiment which was repeated in three biological replicates. For details, see Materials and Methods.bControl strain containing vector pSUP202pol4 chromosomally integrated downstream of the ΔosrA::aphII locus (see Figure 2B).