Quasispecies analysis of HCVp0 populations passaged in the absence or presence of ribavirina.

aThe population analyzed correspond to passage 3 of the infections at an initial MOI of 0.1 to 0.2 TCID50/cell described in Fig. 3b.bThe HCV genome residue numbering corresponds to the JFH-1 genome (accession number #AB047639). The NS5B-coding region was covered by two overlapping amplifications.cThe parenthesis indicates the number of clones analyzed, followed by the number of haplotypes (number of different RNA sequences); some clones did not contain the full length sequence; when the alignment of the sequenced region was correct such clones were entered in the calculation.dAverage number of mutations per nucleotide relative to the corresponding consensus sequence. Mutation types are summarized in Fig. 4a and their position in the HCV genome and deduced amino acid substitutions are given in Tables S1, S2 and S3.eThe calculation of nucleotide diversity (π) and 95% confidence interval (CI) is explained in Materials and Methods.