Single-cell Definition of the full-term Developmental Program of Male Mouse Germline

Germ cells are essential for the genetic information transition to subsequent generations of the species relying on sexual reproduction. Full-term developmental trajectory of germ cells provides a paradigm for understanding mammalian germline and germline niches with pivotal knowledge from tremendous in vivo and in vitro studies on the critical role of master regulators in regulating the initiation and maintenance of germline.To characterise the dynamic process of transcriptional programme underlying male mouse germline development, we generated single-cell RNA sequencing profiles for 28 developmental time-points from dissected early embryos or male gonads that were collected between embryonic day (E) 6.5 and postnatal day (P) 8 at one-day intervals, P10-P14 at two-day intervals and at juvenile stage of P30 and P35 as well as adult (8-10 weeks). Overall design: A full-term single cell transcriptome profile of mouse germline from PGCs in E6.5 to spermatid in 10w after birth were generated by next generation sequencing using Illumina HiSeq X-Ten.