Transcriptional response to interferon beta and effects of deletion of a predicted enhancer

We test the effect of CRISPR knockout of a predicted interferon inducible enhancer within the first intron of the IFNAR1 gene (IFNAR1.L1M2a) on the transcriptional response of GM12878 cells to treatment with interferon beta Clonal wildtype GM12878 lines and clonal CRISPR knockout lines lacking a predicted enhancer within the first intron of the the IFNAR1 gene were either untreated or treated with 10 units per ml of human interferon beta over a time course of 4, 12, and 24 hours, and then profiled by RNA-seq