Optimization and Clinical Validation of a Pathogen Detection Microarray. Optimization and Clinical Validation of a Pathogen Detection Microarray

This series includes microarrays from 36 patient samples and 2 cell-culture controls, used to optimize and validate the pathogen detection microarray (Wong, et. al. 2007) Keywords: viral pathogen detection Overall design: The microarray contains tiling probes to 35 viruses, and was used to detect the viruses present in 36 patient samples. Viruses were reverse-transcribed and PCR-amplified using either primer A1 or AES-optimized primer A2 as described (Wong , et. al. 2007). Those samples which were amplified using primer A2 are labelled with "A2" in the sample title. Patient samples #122 and #412 were hybridized on multiple arrays (technical replicates). The SARS and Dengue samples were amplified using virus-specific primers as described in Wong, et al. 2007.