Thermal unfolding datasets of two short RNA molecules - DsrA_core_WT and DsrA_core_mut - measured by circular dichroism

This repository contains two thermal unfolding datasets measured by circular dichroism (CD). The RNA sequences are shown below. DsrAcoreWT (pentaloop): 5’-AACACAUCAGAUUUCCUGGUGUAACGAAUUUUUUAAG-3’ DsrAcoremut (tetraloop): 5’-AACACAUCAGGGAACUGGUGUAACGAAUUUUUUAAG-3’ Moreover, a video showing how to analyse the data with the online tool ChiraKit (https://spc.embl-hamburg.de/app/chirakit) is available ('video_fitting_two_state_DsrA_core_mut.mp4'). Briefly, the spectra are first decomposed using singular value decomposition, and the associated coefficients are then fitted to a two-state unfolding model. The CD data was acquired by Florian Turbant under the supervision of Veronique Arluison. The analysis shown in the video was done by Osvaldo Burastero.