Characterization of leaf transcriptome in a tropical tree species, Shorea curtisii, over a flowering season

General flowering (GF) is a synchronous flowering event in the Southeast Asian tropical rainforests that occurs at irregular intervals of multiple years. The unpredictable intervals of GF raise conservation concerns for these under-researched forests with rich economically and ecologically important species. In this study, the leaf transcriptome of a GF species, Shorea curtisii obtained from three time points – before and after floral initiation, and post flowering stage – was sequenced. We assembled 243,759,478 sequencing reads into 39,943 non-redundant unigenes including 677 putative homologs of Arabidopsis thaliana flowering-related genes. Differential expression analysis conducted on pairwise comparisons of the time points identified 930 differentially expressed unigenes, which includes 17 flowering-related homologs. The differential expression of unigenes with significant enrichments of functions related to drought corroborated the involvement of drought as an environmental cue for..., Leaf samples at top layer of canopy from two S. curtisii individuals, C1 and C2 were collected from Semangkok Forest Reserve (2°58’N, 102°18’E). We selected three time points (TPs) corresponding to before and after floral initiation, and abortion/fruiting stage. Samples were collected at each TP around midday, soaked in RNAlater reagent (Ambion, USA), and stored at –80°C.
Total RNA was extracted from leaf samples using the method described by Kobayashi et al. (2013). RNA-seq was performed using Illumina HiSeq 4000 Sequencer (Illumina, USA). After removal of low quality bases by Trimmomatic v0.36 (Bolger et al., 2014), the sequence reads from each sample were combined and de novo assembled using Trinity v2.8.5 (Grabherr et al., 2011). Only non-redundant transcripts with complete open reading frame were retained by using TransDecoder v5.5.0 (http://transdecoder.github.io) and CD-HIT v4.8.1 (Fu et al., 2012). The quality of the assembly was assessed with BUSCO v5.2.1 (Simao et al., 2015). ..., Fig_S1_3_Table_S1_2.pdf: A pdf file which contains Supplementary Figure S1–S3 and Table S1–S2. 
Fig S1 is a flow chart of research strategy employed to study the leaf transcriptome of Shorea curtisii during a flowering season. Details of the methodology are described in the legend in details.
Fig S2 is a histogram which shows the distribution of the contig lengths of S. curtisii transcriptome.
Fig S3 is a bar chart which depicts the classification of S. curtisii unigenes into three main Gene Ontology categories.
Table S1 is a table which contains details of meteorological status, specifically daily minimum, maximum, and mean temperature, as well as rainfall records during sampling days. The records were obtained from the nearest meteorological and hydrological stations to the sampling site. The first line shows titles of data which include information on when the sampling was done i.e. time point, date, developmental stage, and sampling time for individual trees, as well as meteorologic..., # Characterization of leaf transcriptome in a tropical tree species, Shorea curtisii, over a flowering season

[https://doi.org/10.5061/dryad.69p8cz94f](https://doi.org/10.5061/dryad.69p8cz94f)

### Description of the data and file structure

Fig\_S1\_3\_Table\_S1\_2.pdf: A pdf file which contains Supplementary Figure S1-3 and Table S1-2.

* Fig S1 is a flow chart of research strategy employed to study the leaf transcriptome of Shorea curtisii during a flowering season. Details of the methodology is described in the legend in details.
* Fig S2 is a histogram which shows the distribution of the contig lengths of S. curtisii transcriptome.
* Fig S3 is a bar chart which depicts the classification of S. curtisii unigenes into three main Gene Ontology categories.

Table S1 is a table which contains details of meteorological status, specifically daily minimum, maximum, and mean temperature, as well as rainfall records during sampling days. The records were obtained from the nearest meteor...